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zeige Details De Koning, W.., Bonting, K.., ... Classical transketolase functions as the formaldehyde-assimilating enzyme during growth of a dihydroxyacetone synthase-negative mutant of the methylotrophic yeast Hansenula...
in: Yeast, in: Yeast . - New York, NY [u.a.] : Wiley, ISSN 0749-503X, ZDB-ID 1479172-9 Vol. 6 (2. 1990), p. 117-125
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1990
Contrary to expectation, a mutant of Hansenula polymorpha blocked in dihydroxyacetone (DHA) synthase was able to assimilate methanol-carbon when grown in chemostat culture on mixtures of xylose and methanol. Incubation of a DHA synthase- and DHA kinase-negative double mutant resulted in DHA accumulation, indicating that a DHA synthase-type of reaction was involved. Low residual DHA synthase activity subsequently was shown to be present when using an assay with improved sensitivity. This activity was not associated with the (mutated) DHA synthase protein, which was still present in the peroxisomes, but with the enzyme transketolase. Transketolase from methanol grown cells was purified (525-fold) to homogeneity in 9% yield. The native enzyme was dimeric, as has been reported fro other transketolases, with a subunit molecular weight of 74000. The affinity of the purified enzyme for formaldehyde was low (Km = 5 mM), but high for xylulose-5-phosphate (ca. 10 μM). The in vivo functioning of transketolase in formaldehyde assimilation, and the influence of the hydration state of formaldehyde is discussed.
Copyright: Copyright © 1990 John Wiley & Sons Ltd.
beteiligte Personen: De Koning, W.. , Bonting, K.. , Harder, W.. , Dijkhuizen, L..
Format: Elektronisch
Sprache: English
Erschienen: 1990.
Serie: Wiley InterScience Backfile Collection 1832-2000 [Dig. Serial]
Schlagwörter:
URL: http://dx.doi.org/10.1002/yea.320060205

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Verfasser Titel Jahr
zeige Details De Koning, W.., Weusthuis, R. A.., ... Metabolic regulation in the yeast Hansenula polymorpha. Growth of dihydroxyacetone kinase/glycerol kinase-negative mutants on mixtures of methanol and xylose in continuous cultures
in: Yeast, in: Yeast . - New York, NY [u.a.] : Wiley, ISSN 0749-503X, ZDB-ID 1479172-9 Vol. 6 (2. 1990), p. 107-115
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1990
zeige Details Sibirny, A. A., Ubiyvovk, V. M., ... Reactions of direct formaldehyde oxidation to CO2 are non-essential for energy supply of yeast methylotrophic growth
in: Archives of microbiology , ISSN 1432-072X, Vol. 154 (6. 1990), p. 566-575
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1990
zeige Details Yanase, H., Okuda, M., ... Enzymatic preparation of [1, 3-13C]dihydroxyacetone phosphate from [13C]methanol and hydroxypyruvate using the methanol- assimilating system of methylotrophic yeasts
in: Applied microbiology and biotechnology , ISSN 1432-0614, Vol. 43 (2. 1995), p. 228-234
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1995
zeige Details Egli, Th., Käppeli, O., ... Mixed substrate growth of methylotrophic yeasts in chemostat culture: Influence of the dilution rate on the utilisation of a mixture of glucose and methanol
in: Archives of microbiology , ISSN 1432-072X, Vol. 131 (1. 1982), p. 8-13
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1982
zeige Details Yanase, H., Okuda, M., ... Enzymatic preparation of [1, 3-13C]dihydroxyacetone phosphate from [13C]methanol and hydroxypyruvate using the methanol-assimilating system of methylotrophic yeasts
in: Applied microbiology and biotechnology , ISSN 1432-0614, Vol. 43 (2. 1995), p. 228-234
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