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Verfasser Titel Jahr
zeige Details Dall, M. -T., Nicaud, J. -M., ... The 3-phosphoglycerate kinase gene of the yeastYarrowia lipolytica de-represses on gluconeogenic substrates
in: Current genetics , ISSN 1432-0983, Vol. 29 (5. 1996), p. 446-456
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1996
Abstract We have isolated the 3-phosphoglycerate kinase (PGK) gene of the yeastYarrowia lipolytica by probing a genomic library with a PCR fragment amplified with primers deduced from two highly conserved regions of various PGKs. It is a unique sequence encoding a polypeptide of 417 residues with extensive homology to other PGKs, especially to that ofAspergillus nidulans (76% identity). The expression of theY. lipolytica PGK1 gene proved to be higher on gluconeogenic substrates than on glycolytic ones. Haploid strains harboring a disrupted allele were able to grow on mixtures of a gluconeogenic carbon source and of a glycolytic one, but required proline supplementation in the presence of glucose, and were inhibited by glycerol.
Copyright: Copyright 1996 Springer-Verlag
beteiligte Personen: Dall, M. -T. , Nicaud, J. -M. , Tréton, B. Y. , Gaillardin, C. M.
Format: Elektronisch
Erschienen: 1996.
Serie: Springer Online Journal Archives 1860-2000 [Dig. Serial]
Schlagwörter:
URL: http://dx.doi.org/10.1007/BF02221513

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