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zeige Details Nicaud, J. -M., Raynal, A., ... Stabilization of methionine-rich protein in Saccharomyces cerevisiae: targeting of BZN protein into the peroxisome
in: Current genetics , ISSN 1432-0983, Vol. 26 (5/6. 1994), p. 390-397
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1994
Abstract We have constructed a gene coding for the 12-kDa intermediate form of the 2s methionine-rich protein from Bertholletia excelsa seeds. This protein, expressed intracellularly in yeast, is characterised by a 20-min balf-life. By adding 11 amino acids corresponding to the peroxisome-targeting sequence (PTSc) of luciferase, we have significantly increased its half-life. This stabilization allowed accumulation of the BZN protein into the peroxisome as judged by cell fractionation. Accumulation of the 12-kDa protein results in a significant increase of the total methionine content in yeast cells (30%) indicating that such a microorganism could represent a practicable protected shuttl for an animal-feed additive.
Copyright: Copyright 1994 Springer-Verlag
beteiligte Personen: Nicaud, J. -M. , Raynal, A. , Beyou, A. , Merkamm, M. , Ito, H. , Labat, N.
Format: Elektronisch
Erschienen: 1994.
Serie: Springer Online Journal Archives 1860-2000 [Dig. Serial]
Schlagwörter:
URL: http://dx.doi.org/10.1007/BF00309924

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