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zeige Details Pagot, Y., Le Clainche, A., ... Peroxisomal β-oxidation activities and γ-decalactone production by the yeast Yarrowia lipolytica
in: Applied microbiology and biotechnology , ISSN 1432-0614, Vol. 49 (3. 1998), p. 295-300
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1998
Abstract γ-Decalactone is a peachy aroma compound resulting from the peroxisomal β-oxidation of ricinoleic acid by yeasts. The expression levels of acyl-CoA oxidase (gene deletion) and 3-ketoacyl-CoA thiolase activities (gene amplification on replicative plasmids) were modified in the yeast Yarrowia lipolytica. The effects of these modifications on β-oxidation were measured. Overexpression of thiolase activity did not have any effect on the overall β-oxidation activity. The disruption of one of the acyl-CoA oxidase genes resulted in an enhanced activity. The enhancement led to an increase of overall β-oxidation activity but reduced the γ-decalactone production rates. This seemed to indicate a non-rate-limiting role for β-oxidation in the biotransformation of ricinoleic acid to γ-decalactone by the yeast Yarrowia lipolytica. All strains produced and then consumed γ-decalactone. We checked the ability of the different strains to consume γ-decalactone in a medium containing the lactone as sole carbon source. The consumption of the strain overexpressing acyl-CoA oxidase activity was higher than that of the wild-type strain. We␣concluded that peroxisomal β-oxidation is certainly involved in γ-decalactone catabolism by the yeast Y.␣lipolytica. The observed production rates probably depend on an equilibrium between production and consumption of the lactone.
Copyright: Copyright 1998 Springer-Verlag Berlin Heidelberg
beteiligte Personen: Pagot, Y. , Le Clainche, A. , Nicaud, J.-M. , Wache, Y. , Belin, J.-M.
Format: Elektronisch
Erschienen: 1998.
Serie: Springer Online Journal Archives 1860-2000 [Dig. Serial]
Schlagwörter:
URL: http://dx.doi.org/10.1007/s002530051172

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