Anmelden/Registrieren
Zurück zur geteilten Ansicht
Verfasser Titel Jahr
zeige Details Harmon, Fred R., Subrahmanyam, Chirala S., ... Interactions of U1 RNP with heterogeneous nuclear RNA in rat Novikoff hepatoma nuclei
in: Molecular and cellular biochemistry , ISSN 1573-4919, Vol. 65 (1. 1984), p. 45-55
Zugang: zum Volltext
ähnliche Vorschläge
1984
Summary The nature of the association of U1 RNA with rapidly sedimenting RNP structures in rat hepatoma nuclei was investigated. The effects of salt and proteinase K treatment on the stability of this ‘bound’ form of U1 RNA were studied using sucrose density gradient analyses. Quantitation of the amount of U1 RNA remaining associated with large structures after treatment was used to assess the relative contribution of protein-protein(and protein-RNA) versus RNA-RNA interactions. Forty-eight percent of the total nuclear U1 RNA released by sonication was found in a ‘bound’ form when the sonicate was centrifuged through gradients containing 50 mM NaCl. Fifty percent of this ‘bound’ U1 RNA remained associated with rapidly sedimenting RNPs when the NaCl concentration was raised to 500 mM. To assess the contribution of protein independent interactions, large RNPs were completely deproteinized and their RNA moieties were then recentrifuged on gradients. By this analysis, 27% of the U1 RNA originally ‘bound’ to hnRNPs was associated with rapidly sedimenting (>30 S) RNA (at 50 mM NaCl) suggesting their association by RNA-RNA hydrogen bonds. When the concentration of NaCl was 500 mM, 31% of the U1 RNA was associated with large RNA. Hence, approximately 30% of the U1 RNA molecules originally ‘bound’ (or about 15% of the total nuclear U1 RNA) were found to be associated by RNA-RNA hydrogen bonds while the remainder of the binding of U1 RNP to hnRNP was by protein-protein and/or protein-RNA interactions.
Copyright: Copyright 1984 Martinus Nijhoff Publishers
beteiligte Personen: Harmon, Fred R. , Subrahmanyam, Chirala S. , Busch, Harris
Format: Elektronisch
Erschienen: 1984.
Serie: Springer Online Journal Archives 1860-2000 [Dig. Serial]
Schlagwörter:
URL: http://dx.doi.org/10.1007/BF00226018

Ähnliche Vorschläge

Zurück zur geteilten Ansicht
Verfasser Titel Jahr
zeige Details BLANCHARD, J. M., DUCAMP, CH., ... Endogenous protein kinase activity in nuclear RNP particles from HeLa cells
in: Nature . - London [u.a.] : Nature Publising Group, ISSN 1476-4687, Vol. 253, No. 5491 (1975), p. 467-468
Zugang: zum Volltext
ähnliche Vorschläge
1975
zeige Details Schweiger, A., Kostka, G. Preparation in undenatured form of the main protein bound to heterogeneous nuclear RNA in liver and hepatoma cells
in: Molecular biology reports , ISSN 1573-4978, Vol. 3 (5. 1977), p. 353-359
Zugang: zum Volltext
ähnliche Vorschläge
1977
zeige Details Seeber, S., Busch, H. Differences in the primary structure of nucleolar and ribosomal high molecular weight RNA of the Novikoff hepatoma and rat liver, and possible therapeutic implications
in: Journal of cancer research and clinical oncology , ISSN 1432-1335, Vol. 78 (3. 1972), p. 265-281
Zugang: zum Volltext
ähnliche Vorschläge
1972
zeige Details Hillar, M., Przyjemski, J. Nuclear deprimerones in rat liver and Novikoff hepatoma
in: Molecular biology reports , ISSN 1573-4978, Vol. 6 (2. 1980), p. 67-71
Zugang: zum Volltext
ähnliche Vorschläge
1980
zeige Details Du, Hansen, Rosbash, Michael The U1 snRNP protein U1C recognizes the 5′ splice site in the absence of base pairing
in: Nature . - London [u.a.] : Nature Publising Group, ISSN 1476-4687, Vol. 419, No. 6902 (2002), p. 86-90
Zugang: zum Volltext
ähnliche Vorschläge
2002