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zeige Details Lahiri, Debomoy K., Schnabel, Bill DNA isolation by a rapid method from human blood samples: Effects of MgCl2, EDTA, storage time, and temperature on DNA yield and quality
in: Biochemical genetics , ISSN 1573-4927, Vol. 31 (7/8. 1993), p. 321-328
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1993
Abstract The isolation of DNA from whole blood by a modified rapid method (RM) was tested using various detergents and buffer conditions. Extraction of DNA with either NP-40 or Triton X-100 gave a high yield of undegraded DNA in less than an hour. The concentration of magnesium ion in the buffers was critical to obtaining intact, high molecular weight (HMW) DNA. Greater than 10 mM MgCl2 led to degradation. Addition of EDTA to the buffer inhibits this degradation. Preparation of DNA from blood stored at room temperature or incubated at 37°C for 24 hr resulted in the same amount and quality of DNA as from samples frozen at −70°C. DNA from blood samples that had undergone more than four freeze-thaw cycles was found to be partially degraded. The modified RM can be applied to extract DNA from as little as 10 µl of blood (340 ng of DNA) and from dried blood samples. DNA samples remained intact and undegraded for longer times when DNA was dissolved in higher concentrations of EDTA.
Copyright: Copyright 1993 Plenum Publishing Corporation
beteiligte Personen: Lahiri, Debomoy K. , Schnabel, Bill
Format: Elektronisch
Erschienen: 1993.
Serie: Springer Online Journal Archives 1860-2000 [Dig. Serial]
Schlagwörter:
URL: http://dx.doi.org/10.1007/BF02401826

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Verfasser Titel Jahr
zeige Details Lahiri, Debomoy K., Schnabel, Bill DNA isolation by a rapid method from human blood samples: Effects of MgCl2, EDTA, storage time, and temperature on DNA yield and quality
in: Biochemical genetics , ISSN 1573-4927, Vol. 31 (7/8. 1993), p. 321-328
Zugang: zum Volltext
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