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zeige Details Altenbach, Susan B., Pearson, Karen W., ... Enhancement of the methionine content of seed proteins by the expression of a chimeric gene encoding a methionine-rich protein in transgenic plants
in: Plant molecular biology , ISSN 1573-5028, Vol. 13 (5. 1989), p. 513-522
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1989
Abstract We have constructed a chimeric gene encoding a Brazil nut methionine-rich seed protein which contains 18% methionine. This gene has been transferred to tobacco and expressed in the developing seeds. Tobacco seeds are able to process the methionine-rich protein efficiently from a larger precursor polypeptide of 17 kDa to the 9kDa and 3 kDa subunits of the mature protein, a procedure which involves three proteolytic cleavage steps in the Brazil nut seed. The accumulation of the methionine-rich protein in the seeds of tobacco results in a significant increase (30%) in the levels of the methionine in the seed proteins of the transgenic plants. Our data indicate that the introduction of a chimeric gene encoding a methionine-rich seed protein into crop plants, particularly legumes whose seeds are deficient in the essential sulfur-containing amino acids, represents a feasible method for improving the nutritional quality of seed proteins.
Copyright: Copyright 1989 Kluwer Academic Publishers
beteiligte Personen: Altenbach, Susan B. , Pearson, Karen W. , Meeker, Gabrielle , Staraci, Lisa C. , Sun, Samuel S. M.
Format: Elektronisch
Erschienen: 1989.
Serie: Springer Online Journal Archives 1860-2000 [Dig. Serial]
Schlagwörter:
URL: http://dx.doi.org/10.1007/BF00027311

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