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zeige Details Cole, D. G., Chinn, S. W., ... Novel heterotrimeric kinesin-related protein purified from sea urchin eggs
in: Nature . - London [u.a.] : Nature Publising Group, ISSN 1476-4687, Vol. 366, No. 6452 (1993), p. 268-270
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1993
KINESIN heavy chain and kinesin-related polypeptides (KRPs)comprise a family of motor proteins with diverse intracellular transportfunctions1–7. Using pan-kinesin peptide antibodies thatreact with these proteins8,9, we haven previously purified fromsea urchin eggs a trimeric microtubule-binding and bundling protein,KRP(85/95) (ref. 8) comprising subunits ofMr115,000 (115K), 95K and 85K. We report here thatkinesin-related genes encode the 85K and 95K subunits, and that the protein canbe immunoprecipitated from cytosol as a trimeric complex using an 85Kmonoclonal antibody. We also find that purified KRP(85/95) directsmovements towards the 'plus' ends of microtubules. To our knowledge, thisprotein is the first kinesin-related motor to be purified from its natural hostcell in a native multimeric state.
beteiligte Personen: Cole, D. G. , Chinn, S. W. , Wedaman, K. P. , Hall, K. , Vuong , T. , Scholey, J. M.
Format: Elektronisch
Erschienen: 1993.
Serie: Nature Archives 1869 - 2007 [Dig. Serial]
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URL: http://dx.doi.org/10.1038/366268a0

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